Determination of the Concentration of IgG against the Spike Receptor-Binding Domain That Predicts the Viral Neutralizing Activity of Convalescent Plasma and Serum against SARS-CoV-2

Santiago, Llipsy ; Uranga-Murillo, Iratxe ; Arias, Maykel ; González-Ramírez, Andrés M. (Universidad de Zaragoza) ; Macías-León, Javier (Universidad de Zaragoza) ; Moreo, Eduardo (Universidad de Zaragoza) ; Redrado, Sergio ; García-García, Ana (Universidad de Zaragoza) ; Taleb, Víctor (Universidad de Zaragoza) ; Lira-Navarrete, Erandi (Universidad de Zaragoza) ; Hurtado-Guerrero, Ramón ; Aguilo, Nacho (Universidad de Zaragoza) ; del Mar Encabo-Berzosa, Maria ; Hidalgo, Sandra ; Galvez, Eva M. ; Ramirez-Labrada, Ariel ; de Miguel, Diego ; Benito, Rafael (Universidad de Zaragoza) ; Miranda, Patricia ; Fernández, Antonio (Universidad de Zaragoza) ; Domingo, José M. ; Serrano, Laura ; Yuste, Cristina ; Villanueva-Saz, Sergio (Universidad de Zaragoza) ; Paño-Pardo, José R. (Universidad de Zaragoza) ; Pardo, Julián (Universidad de Zaragoza)

Resumen: Several hundred millions of people have been diagnosed of coronavirus disease 2019 (COVID-19), causing millions of deaths and a high socioeconomic burden. SARS-CoV-2, the causative agent of COVID-19, induces both specific T- and B-cell responses, being antibodies against the virus detected a few days after infection. Passive immunization with hyperimmune plasma from convalescent patients has been proposed as a potentially useful treatment for COVID-19. Using an in-house quantitative ELISA test, we found that plasma from 177 convalescent donors contained IgG antibodies specific to the spike receptor-binding domain (RBD) of SARS-CoV-2, although at very different concentrations which correlated with previous disease severity and gender. Anti-RBD IgG plasma concentrations significantly correlated with the plasma viral neutralizing activity (VN) against SARS-CoV-2 in vitro. Similar results were found using an independent cohort of serum from 168 convalescent health workers. These results validate an in-house RBD IgG ELISA test in a large cohort of COVID-19 convalescent patients and indicate that plasma from all convalescent donors does not contain a high enough amount of anti-SARS-CoV-2-RBD neutralizing IgG to prevent SARS-CoV-2 infection in vitro. The use of quantitative anti-RBD IgG detection systems might help to predict the efficacy of the passive immunization using plasma from patients recovered from SARS-CoV-2.

Idioma: Inglés

DOI: 10.3390/biology10030208

Año: 2021

Publicado en: Biology 10, 3 (2021), 208 [14 pp.]
ISSN: 2079-7737

Factor impacto JCR: 5.168 (2021)

Categ. JCR: BIOLOGY rank: 21 / 94 = 0.223 (2021) - Q1 - T1

Factor impacto CITESCORE: 2.8 - Agricultural and Biological Sciences (Q2) - Immunology and Microbiology (Q3) - Biochemistry, Genetics and Molecular Biology (Q3)

Factor impacto SCIMAGO: 0.903 - Biochemistry, Genetics and Molecular Biology (miscellaneous) (Q1) - Agricultural and Biological Sciences (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA-FEDER/B29-17R
Financiación: info:eu-repo/grantAgreement/ES/DGA-FEDER/E34-R17
Financiación: info:eu-repo/grantAgreement/ES/DGA/LMP58-18
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/COV20-00308
Financiación: info:eu-repo/grantAgreement/ES/MCIU-AEI/SAF2017-83120-C2-1-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BFU2016-75633-P
Financiación: info:eu-repo/grantAgreement/ES/MINECO/CTQ2013-44367-C2-2-P
Financiación: info:eu-repo/grantAgreement/ES/MINECO/PID2019-105451GB-I00

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Microbiología (Dpto. Microb.Ped.Radio.Sal.Pú.)
Área (Departamento): Area Medicina (Dpto. Medicina, Psiqu. y Derm.)
Área (Departamento): Área Inmunología (Dpto. Microb.Ped.Radio.Sal.Pú.)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)