Post-incubation pH Impacts the Lipid Extraction Assisted by Pulsed Electric Fields from Wet Biomass of "Auxenochlorella protothecoides"

Delso, Carlota (Universidad de Zaragoza) ; Silve, Aude ; Wüstner, Rüdiger ; Nazarova, Natalja ; Álvarez, Ignacio (Universidad de Zaragoza) ; Raso, Javier (Universidad de Zaragoza) ; Frey, Wolfgang

Resumen: Pulsed electric field (PEF) treatment is a promising technology for efficient lipid extraction from microalgae. This study focusses on under-investigated processing parameters, such as media pH, pulse application, and incubation protocols. The lipid yield and electroporation level of PEF-treated Auxenochlorella protothecoides were determined at a medium pH of 3.0 and 5.0 under variation of the pre- or post-PEF incubation time and for split-dose treatments. Low energetic PEF treatments with 40 kV/cm and 1 μs pulses at 9.6 and 19.2 kJ/L were performed either in batch mode or in continuous flow. Post-PEF incubation significantly increased the shared electroporated cells (>60%) in medium pH 3.0, while no change was observed at pH 5.0. Split-dose PEF treatments at pH 5.0 caused significantly higher electroporation levels and lipid extraction yields than equivalent single-dose treatments. Results have shown that medium pH is critical in the final electroporation and lipid extraction yields of A. protothecoides and therefore should be considered in further studies.

Idioma: Inglés

DOI: 10.1021/acssuschemeng.2c04016

Año: 2022

Publicado en: ACS Sustainable Chemistry and Engineering 10, 37 (2022), 12448-12456
ISSN: 2168-0485

Factor impacto JCR: 8.4 (2022)

Categ. JCR: ENGINEERING, CHEMICAL rank: 13 / 141 = 0.092 (2022) - Q1 - T1
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 32 / 178 = 0.18 (2022) - Q1 - T1
Categ. JCR: GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY rank: 14 / 46 = 0.304 (2022) - Q2 - T1

Factor impacto CITESCORE: 15.3 - Environmental Science (Q1) - Chemistry (Q1) - Chemical Engineering (Q1) - Energy (Q1)

Factor impacto SCIMAGO: 1.744 - Chemical Engineering (miscellaneous) (Q1) - Renewable Energy, Sustainability and the Environment (Q1) - Environmental Chemistry (Q1) - Chemistry (miscellaneous) (Q1)

Tipo y forma: Article (Published version)

Área (Departamento): Área Tecnología de Alimentos (Dpto. Produc.Animal Cienc.Ali.)