Freezing preservation procedure of caecal inoculum for microbial fermentation studies in pigs
Amanzougarene, Zahia ; Pérez-Calvo, Estefanía ; Fondevila, Manuel (Universidad de Zaragoza)
Resumen: Aim of study: To define freezing conditions that preserve fermentative capacity of microbial inoculum for in vitro studies in pigs.
Material and methods: Caecal contents from three slaughtered pigs were obtained for being used as inoculum. Part of it was immediately frozen in liquid N and stored at -80ºC, whereas the rest was directly used as fermentation inocula. Incubation substrate was pre-digested in pepsin and pancreatin to simulate the processes occurring before the caecum. Pre-digested substrate was incubated alone or supplemented by three additives consisting of two commercial additives based on essential oils mixtures (CRINA-TEP and CRINA-TMEC) and riboflavin. Gas production at 2, 4, 6, 8, 10, and 12 h, and methane, short chain fatty acids (SCFA), and ammonia concentration at 6 h and 12 h were recorded. .
Idioma: Inglés
DOI: 10.5424/sjar/2022203-19272
Año: 2022
Publicado en: Spanish Journal of Agricultural Research 20, 3 (2022), 7
ISSN: 1695-971X
Factor impacto JCR: 0.9 (2022)
Categ. JCR: AGRICULTURE, MULTIDISCIPLINARY rank: 40 / 58 = 0.69 (2022) - Q3 - T3
Factor impacto CITESCORE: 1.9 - Agricultural and Biological Sciences (Q3)
Factor impacto SCIMAGO: 0.249 - Agronomy and Crop Science (Q3)
Tipo y forma: Article (Published version)
Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Exportado de SIDERAL (2024-03-18-15:39:54)
Visitas y descargas
Material and methods: Caecal contents from three slaughtered pigs were obtained for being used as inoculum. Part of it was immediately frozen in liquid N and stored at -80ºC, whereas the rest was directly used as fermentation inocula. Incubation substrate was pre-digested in pepsin and pancreatin to simulate the processes occurring before the caecum. Pre-digested substrate was incubated alone or supplemented by three additives consisting of two commercial additives based on essential oils mixtures (CRINA-TEP and CRINA-TMEC) and riboflavin. Gas production at 2, 4, 6, 8, 10, and 12 h, and methane, short chain fatty acids (SCFA), and ammonia concentration at 6 h and 12 h were recorded. .
Idioma: Inglés
DOI: 10.5424/sjar/2022203-19272
Año: 2022
Publicado en: Spanish Journal of Agricultural Research 20, 3 (2022), 7
ISSN: 1695-971X
Factor impacto JCR: 0.9 (2022)
Categ. JCR: AGRICULTURE, MULTIDISCIPLINARY rank: 40 / 58 = 0.69 (2022) - Q3 - T3
Factor impacto CITESCORE: 1.9 - Agricultural and Biological Sciences (Q3)
Factor impacto SCIMAGO: 0.249 - Agronomy and Crop Science (Q3)
Tipo y forma: Article (Published version)
Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. Exportado de SIDERAL (2024-03-18-15:39:54)
Permalink:
Visitas y descargas
Este artículo se encuentra en las siguientes colecciones:
Articles > Artículos por área > Producción Animal
Record created 2022-11-24, last modified 2024-03-19