Freezing preservation procedure of caecal inoculum for microbial fermentation studies in pigs

Amanzougarene, Zahia ; Pérez-Calvo, Estefanía ; Fondevila, Manuel (Universidad de Zaragoza)

Resumen: Aim of study: To define freezing conditions that preserve fermentative capacity of microbial inoculum for in vitro studies in pigs.
Material and methods: Caecal contents from three slaughtered pigs were obtained for being used as inoculum. Part of it was immediately frozen in liquid N and stored at -80ºC, whereas the rest was directly used as fermentation inocula. Incubation substrate was pre-digested in pepsin and pancreatin to simulate the processes occurring before the caecum. Pre-digested substrate was incubated alone or supplemented by three additives consisting of two commercial additives based on essential oils mixtures (CRINA-TEP and CRINA-TMEC) and riboflavin. Gas production at 2, 4, 6, 8, 10, and 12 h, and methane, short chain fatty acids (SCFA), and ammonia concentration at 6 h and 12 h were recorded. .

Idioma: Inglés

DOI: 10.5424/sjar/2022203-19272

Año: 2022

Publicado en: Spanish Journal of Agricultural Research 20, 3 (2022), 7
ISSN: 1695-971X

Factor impacto JCR: 0.9 (2022)

Categ. JCR: AGRICULTURE, MULTIDISCIPLINARY rank: 40 / 58 = 0.69 (2022) - Q3 - T3

Factor impacto CITESCORE: 1.9 - Agricultural and Biological Sciences (Q3)

Factor impacto SCIMAGO: 0.249 - Agronomy and Crop Science (Q3)

Tipo y forma: Article (Published version)

Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)