Poly-e-Caprolactone/Fibrin-Alginate scaffold: a new Pro-Angiogenic composite biomaterial for the treatment of bone defects

Ren, Jiongyu ; Kohli, Nupur ; Sharma, Vaibhav ; Shakouri, Taleen ; Keskin-Erdogan, Zalike ; Saifzadeh, Siamak ; Brierly, Gary I. ; Knowles, Jonathan C. ; Woodruff, Maria A. ; García-Gareta, Elena

Resumen: We hypothesized that a composite of 3D porous melt-electrowritten poly-ɛ-caprolactone (PCL) coated throughout with a porous and slowly biodegradable fibrin/alginate (FA) matrix would accelerate bone repair due to its angiogenic potential. Scanning electron microscopy showed that the open pore structure of the FA matrix was maintained in the PCL/FA composites. Fourier transform infrared spectroscopy and differential scanning calorimetry showed complete coverage of the PCL fibres by FA, and the PCL/FA crystallinity was decreased compared with PCL. In vitro cell work with osteoprogenitor cells showed that they preferentially bound to the FA component and proliferated on all scaffolds over 28 days. A chorioallantoic membrane assay showed more blood vessel infiltration into FA and PCL/FA compared with PCL, and a significantly higher number of bifurcation points for PCL/FA compared with both FA and PCL. Implantation into a rat cranial defect model followed by microcomputed tomography, histology, and immunohistochemistry after 4- and 12-weeks post operation showed fast early bone formation at week 4, with significantly higher bone formation for FA and PCL/FA compared with PCL. However, this phenomenon was not extrapolated to week 12. Therefore, for long-term bone regeneration, tuning of FA degradation to ensure syncing with new bone formation is likely necessary.

Idioma: Inglés

DOI: 10.3390/polym13193399

Año: 2021

Publicado en: Polymers 13, 19 (2021), 3399 [22 pp.]
ISSN: 2073-4360

Factor impacto JCR: 4.967 (2021)

Categ. JCR: POLYMER SCIENCE rank: 16 / 90 = 0.178 (2021) - Q1 - T1

Factor impacto CITESCORE: 5.7 - Materials Science (Q1)

Factor impacto SCIMAGO: 0.726 - Polymers and Plastics (Q1) - Chemistry (miscellaneous) (Q1)

Tipo y forma: Article (Published version)