From Bench to Cell: A Roadmap for Assessing the Bioorthogonal "Click" Reactivity of Magnetic Nanoparticles for Cell Surface Engineering

Idiago-Lopez, Javier ; Moreno-Antolin, Eduardo ; Eceiza, Maite ; Aizpurua, Jesus M. ; Grazu, Valeria ; Martínez de la Fuente, Jesús ; Fratila, Raluca M.

Resumen: In this work, we report the use of bioorthogonal chemistry, specifically the strain-promoted click azide-alkyne cyclo-addition (SPAAC) for the covalent attachment of magnetic nano-particles (MNPs) on living cell membranes. Four types of MNPs were prepared, functionalized with two different stabilizing/passivation agents (a polyethylene glycol derivative and a glucopyranoside derivative, respectively) and two types of strained alkynes with different reactivities: a cyclooctyne (CO) derivative and a dibenzocyclooctyne (DBCO) derivative. The MNPs were extensively characterized in terms of physicochemical characteristics, colloidal stability, and click reactivity in suspension. Then, the reactivity of the MNPs toward azide-modified surfaces was evaluated as a closer approach to their final application in a living cell scenario. Finally, the DBCO-modified MNPs, showing superior reactivity in suspension and on surfaces, were selected for cell membrane immobilization via the SPAAC reaction on the membranes of cells engineered to express azide artificial reporters. Overall, our work provides useful insights into the appropriate surface engineering of nanoparticles to ensure a high performance in terms of bioorthogonal reactivity for biological applications.

Idioma: Inglés

DOI: 10.1021/acs.bioconjchem.2c00230

Año: 2022

Publicado en: Bioconjugate Chemistry 33 (2022), 1620-1633
ISSN: 1043-1802

Factor impacto JCR: 4.7 (2022)

Categ. JCR: CHEMISTRY, ORGANIC rank: 8 / 52 = 0.154 (2022) - Q1 - T1
Categ. JCR: BIOCHEMICAL RESEARCH METHODS rank: 13 / 77 = 0.169 (2022) - Q1 - T1
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 61 / 178 = 0.343 (2022) - Q2 - T2
Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 92 / 285 = 0.323 (2022) - Q2 - T1

Factor impacto CITESCORE: 10.1 - Engineering (Q1) - Biochemistry, Genetics and Molecular Biology (Q1) - Chemistry (Q1) - Chemical Engineering (Q1) - Pharmacology, Toxicology and Pharmaceutics (Q1)

Factor impacto SCIMAGO: 1.204 - Bioengineering (Q1) - Biomedical Engineering (Q1) - Pharmacology (Q1) - Organic Chemistry (Q1) - Pharmaceutical Science (Q1) - Biotechnology (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E15-20R
Financiación: info:eu-repo/grantAgreement/EUR/M-ERA.NET/COFUND-MagicCellGene-PCIN-2017-060
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PGC2018-096016-B-I00
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2017-84246-C2-1-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/RYC-2015-17640

Tipo y forma: Artículo (PostPrint)