Combinatorial wound dressings loaded with synergistic antibiotics in the treatment of chronic infected wounds

Miranda-Calderon, Laura (Universidad de Zaragoza) ; Yus, Cristina (Universidad de Zaragoza) ; Remirez de Ganuza, Cristina (Universidad de Zaragoza) ; Paesa, Monica (Universidad de Zaragoza) ; Landa, Guillermo (Universidad de Zaragoza) ; Tapia, Elena (Universidad de Zaragoza) ; Pérez, Estela (Universidad de Zaragoza) ; Perez, Marta (Universidad de Zaragoza) ; Sebastian, Victor (Universidad de Zaragoza) ; Irusta, Silvia (Universidad de Zaragoza) ; Mendoza, Gracia ; Arruebo, Manuel (Universidad de Zaragoza)

Resumen: Advanced medicated wound dressings fabricated by electrospinning and electrospraying were prepared for the eradication of topical bacterial infections potentially applied in the management of infected acute and chronic non-healing wounds. Two different antibiotics (ciprofloxacin and rifampicin), with different aqueous solubilities and different mechanisms of antimicrobial action, were loaded within electrosprayed polymer microparticles and within electrospun nanofibers, respectively, to provide the resulting wound dressing with dually controlled antibiotic release kinetics. Due to the large surface area per volume ratio of the electrosprayed microparticles containing ciprofloxacin, an initial burst release was obtained. Simultaneously, the reduced surface area per volume ratio for the electrospun nanofibers together with the reduced aqueous solubility of rifampicin produced an extended rifampicin release over time. More importantly, a synergistic antimicrobial effect against Gram-positive and Gram-negative bacteria was observed when both antibiotics were combined. Biofilm formation prevention and the elimination of already formed mature bacterial biofilms were also successfully achieved using our advanced dressings. The lack of cytotoxicity of the advanced wound dressings here reported against eukaryotic cells at antimicrobial doses was also demonstrated using three different mammalian cell lines. Moreover, the advanced wound dressings successfully eliminated a Staphylococcus aureus mediated experimental infection in a chronic wound murine model showing their efficacy for the treatment of these complicated non-healing wounds. The strategy of advanced medicated wound dressings developed here may be used as a potential methodology for the fabrication of functional combinatorial materials that offer the ability to eradicate bacterial infections.

Idioma: Inglés

DOI: 10.1016/j.cej.2023.146679

Año: 2023

Publicado en: Chemical Engineering Journal 476 (2023), 146679 [15 pp.]
ISSN: 1385-8947

Factor impacto JCR: 13.4 (2023)

Categ. JCR: ENGINEERING, ENVIRONMENTAL rank: 3 / 81 = 0.037 (2023) - Q1 - T1
Categ. JCR: ENGINEERING, CHEMICAL rank: 7 / 170 = 0.041 (2023) - Q1 - T1

Factor impacto CITESCORE: 21.7 - Environmental Chemistry (Q1) - Industrial and Manufacturing Engineering (Q1) - Chemistry (all) (Q1) - Chemical Engineering (all) (Q1)

Factor impacto SCIMAGO: 2.852 - Environmental Chemistry (Q1) - Industrial and Manufacturing Engineering (Q1) - Chemistry (miscellaneous) (Q1) - Chemical Engineering (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/AEI/PDC2021-121105-I00
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/MS19-00092
Financiación: info:eu-repo/grantAgreement/ES/MCINN/PRE2018-085769
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2020-113987RB-I00

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)
Área (Departamento): Área Anatom.Anatom.Patológ.Com (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)
Área (Departamento): Servicios. División Biomédica (Serv.Gral. Apoyo Investigación)
Área (Departamento): Área Tecnologi. Medio Ambiente (Dpto. Ing.Quím.Tecnol.Med.Amb.)