Linking Exosomal MicroRNA Signatures in Sputum and Peripheral Blood to Inflammatory Endotypes in Severe Asthma

Sanz-Rubio, D. ; Vera, E. ; Rodríguez-Sanz, J. ; Cubero, J. ; Langarita, R. ; Aguado, M. ; Pastor, L. ; Martín, L. ; Marín-Oto, M. ; Remacha, A. R. ; Ruiz, M. ; Gil, V. ; Domingo, J. A. ; Carretero, J. A. ; Marín, J. M.

Resumen: This study is the first to identify and validate distinct exosomal miRNA signatures in both plasma and induced sputum that differentiate T2-high and T2-low endotypes in severe asthma. By integrating miRNA expression with clinical parameters, we developed predictive models with high discriminatory power, highlighting the potential of these miRNAs as non-invasive biomarkers. These findings offer new insights into the molecular basis of asthma endotypes and support a precision medicine approach. In this revised version, guided by the reviewers’ comments, we have improved the clarity of our manuscript by including a new version of Figure 1 with adjusted graphs and greater consistency across panels. We have also added methodological clarifications to enhance the understanding of the experimental workflow, such as the selection criteria for the miRNAs included in the validation step. Additionally, we have completed the clinical data tables to reflect other relevant aspects, such as neutrophil counts and oral corticosteroid use. We are grateful for the opportunity to resubmit this work and for the reviewers’ efforts, which have helped us strengthen several aspects of our manuscript.

Idioma: Inglés

DOI: 10.1111/all.16578

Año: 2025

Publicado en: Allergy: European Journal of Allergy and Clinical Immunology 80, 7 (2025), 2073-2076
ISSN: 0105-4538

Financiación: info:eu-repo/grantAgreement/ES/DGA/B22-23R
Financiación: info:eu-repo/grantAgreement/ES/DGA-CUS/1638-2022
Financiación: info:eu-repo/grantAgreement/ES/ISCIII CD22-00033

Tipo y forma: Article (PostPrint)