Genotypic and phenotypic characterization of methicillin-resistant Staphylococcus aureus (MRSA) clones with high-level mupirocin resistance

González-Domínguez, Maria ; Seral, Cristina (Universidad de Zaragoza) ; Potel, Carmen ; Sáenz, Yolanda ; Alvarez, Maximiliano ; Torres, Carmen ; Castillo, Francisco Javier (Universidad de Zaragoza)

Resumen: An elevated proportion (27.2%) of high-level mupirocin-resistant methicillin-resistant Staphylococcus aureus (HLMUPR-MRSA) isolates were found in our environment in one year period. HLMUPR-MRSA isolates were mainly collected from skin and soft tissue samples, and diabetes was the main related comorbidity condition. These isolates were more frequently found in vascular surgery. HLMUPR-MRSA were more resistant to aminoglycosides than mupirocin-susceptible MRSA, linked to the presence of bifunctional and/or nucleotidyltransferase enzymes with/without macrolide resistance associated with the msr(A) gene. Most of HLMUPR-MRSA isolates belonged to ST125/t067. Nine IS257-ileS2 amplification patterns (p3 was the most frequent) were observed in HLMUPR-MRSA isolates, suggesting the presence of several mupirocin-resistance-carrying plasmids in our environment and promoting the emergence of mupirocin resistance. The presence of the same IS257-ileS2 amplification pattern p3 in 65% of HLMUPR-MRSA, all of them ST125/t067, suggests a clonal spread in our hospital and community environment which could explain the high prevalence of HLMUPR-MRSA during the study period. An outbreak situation or an increase in mupirocin consumption was not observed.

Idioma: Inglés

DOI: 10.1016/j.diagmicrobio.2016.02.021

Año: 2016

Publicado en: Diagnostic Microbiology and Infectious Disease 85 (2016), 213-217
ISSN: 0732-8893

Factor impacto JCR: 2.401 (2016)

Categ. JCR: MICROBIOLOGY rank: 64 / 124 = 0.516 (2016) - Q3 - T2
Categ. JCR: INFECTIOUS DISEASES rank: 47 / 84 = 0.56 (2016) - Q3 - T2

Factor impacto SCIMAGO: 1.237 - Medicine (miscellaneous) (Q1) - Microbiology (medical) (Q2) - Infectious Diseases (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/B24-211130

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Microbiología (Dpto. Microb.Med.Pr.,Sal.Públ.)