Detection and characterization of biogenic selenium nanoparticles in selenium-rich yeast by single particle ICPMS

Jiménez-Lamana, J. ; Abad-Álvaro, I. ; Bierla, K. ; Laborda, F. (Universidad de Zaragoza) ; Szpunar, J. ; Lobinski, R.

Resumen: A method based on single particle inductively coupled plasma mass spectrometry (SP-ICPMS) was developed for the analysis of commercial Se-rich yeasts, to confirm the occurrence of selenium nanoparticles in these food supplements. A considerable reduction of background levels was achieved by combining data acquisition at microsecond dwell times and the use of a H2 reaction cell, improving by a factor of 10 the current state-of-the-art methodology, and bringing size detection limits down to 18 nm for selenium nanoparticles. The presence of nanoparticulate selenium was revealed by size-exclusion chromatography ICPMS, with detection of a selenium peak at the exclusion volume of the column showing absorption at a wavelength corresponding to selenium nanoparticles. SP-ICPMS allowed us to confirm the presence of Se-nanoparticles, as well as to calculate the nanoparticle size distribution, from information about the shape and elemental composition of the nanoparticles obtained by transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDS), respectively. These results reveal the significance of nanoparticles in the speciation of metals and metalloids in biological samples and the capability of SP-ICPMS in combination with TEM-EDS to carry out these analyses.

Idioma: Inglés

DOI: 10.1039/c7ja00378a

Año: 2018

Publicado en: Journal of Analytical Atomic Spectrometry 33, 3 (2018), 452-460
ISSN: 0267-9477

Factor impacto JCR: 3.646 (2018)

Categ. JCR: SPECTROSCOPY rank: 4 / 41 = 0.098 (2018) - Q1 - T1
Categ. JCR: CHEMISTRY, ANALYTICAL rank: 16 / 84 = 0.19 (2018) - Q1 - T1

Factor impacto SCIMAGO: 0.901 - Spectroscopy (Q1) - Analytical Chemistry (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MINECO-FEDER/CTQ2015-68094-C2-1-R

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)