Antifungal activity of the volatile organic compounds produced by Bacillus velezensis strains against postharvest fungal pathogens
Resumen: It is known that Volatile Organic Compounds (VOCs), among several other mechanisms, are responsible for the antagonistic activity produced by microorganisms. In this work the volatilome of three biocontrol Bacillus velezensis strains (BUZ-14, I3 and I5) was tested in vitro and on fruit against Botrytis cinerea, Monilinia fructicola, M. laxa, Penicillium italicum, P. digitatum and P. expansum. In vitro, pathogens growth was significantly inhibited, in particular M. laxa, M. fructicola and P. italicum (66, 72 and 80 %, respectively) by BUZ-14 and B. cinerea (100 %) by I3 and I5, compared to the control. In vivo tests also showed significant inhibitions since volatile metabolites of I3 reduced grey mould in grapes by 50 % and those of BUZ-14 decreased brown rot severity in apricots, especially by M. fructicola, from 60 to 4 mm. VOCs were identified by solid phase microextraction (SPME) coupled with Gas Chromatography-Mass Spectrometry (GC–MS) and the antifungal activity of some of them was tested both in vitro and in vivo against the fungal pathogens. The main volatiles identified ranged from 12 to 15 compounds including 2-nonanone, 2-undecanone, 2-heptanone, 1-butanol, acetoin, benzaldehyde, butyl formate, diacetyl, nonane, or pyrazine, among others. Benzaldehyde and diacetyl obtained the lowest minimum inhibitory concentrations in vitro, ranging from 0.005 to 0.125 mL L-1 depending on the pathogen tested. Moreover, diacetyl was able to control grey mould caused by B. cinerea in table grapes with only 0.02 mL L-1 and to reduce blue rot in mandarins at the same dose up to 60 %. In this study, diacetyl and benzaldehyde have been identified as promising compounds to apply in active packaging during the postharvest commercialization of fruit. However, prior to the application, it is crucial to determine not only the active dose but also the phytotoxic, since some fruit species such as apples and apricots have proven to be highly sensitive.
Idioma: Inglés
DOI: 10.1016/j.postharvbio.2020.111208
Año: 2020
Publicado en: Postharvest Biology and Technology 166 (2020), 111208 [10 pp.]
ISSN: 0925-5214

Factor impacto JCR: 5.537 (2020)
Categ. JCR: FOOD SCIENCE & TECHNOLOGY rank: 20 / 144 = 0.139 (2020) - Q1 - T1
Categ. JCR: AGRONOMY rank: 6 / 91 = 0.066 (2020) - Q1 - T1
Categ. JCR: HORTICULTURE rank: 3 / 37 = 0.081 (2020) - Q1 - T1

Factor impacto SCIMAGO: 1.397 - Agronomy and Crop Science (Q1) - Horticulture (Q1) - Food Science (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/LMP130-18
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Área Nutrición Bromatología (Dpto. Produc.Animal Cienc.Ali.)
Área (Departamento): Área Tecnología de Alimentos (Dpto. Produc.Animal Cienc.Ali.)


Creative Commons Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. No puede utilizar el material para una finalidad comercial. Si remezcla, transforma o crea a partir del material, no puede difundir el material modificado.


Exportado de SIDERAL (2021-09-02-09:14:11)


Visitas y descargas

Este artículo se encuentra en las siguientes colecciones:
Artículos



 Registro creado el 2020-06-16, última modificación el 2021-09-02


Versión publicada:
 PDF
Valore este documento:

Rate this document:
1
2
3
 
(Sin ninguna reseña)