Antimicrobial resistance among canine enteric Escherichia coli isolates and prevalence of attaching-effacing and extraintestinal pathogenic virulence factors in Spain

Sevilla, E. ; Mainar-Jaime, R.C. (Universidad de Zaragoza) ; Moreno, B. (Universidad de Zaragoza) ; Martin-Burriel, I. (Universidad de Zaragoza) ; Morales, M. (Universidad de Zaragoza) ; Andres-Lasheras, S. ; Chirino-Trejo, M. ; Badiola, J.J. (Universidad de Zaragoza) ; Bolea, R. (Universidad de Zaragoza)

Resumen: The aim of this study was to estimate the prevalence of antimicrobial resistance (AMR) in Escherichia coli from a dog population in Spain and assess specific virulence factors. Susceptibility to 22 antimicrobials was tested along with the production of extended-spectrum beta-lactamases (ESBLs) and AmpC in faecal isolates from 100 dogs. Virulence-related genes associated with attaching and effacing E. coli (eae, Stx1, Stx2) and extraintestinal pathogenic E. coli - ExPEC - (papC, hlyA and cnf1) were detected by PCR. At least one kind of AMR was observed in 73% of the isolates. The highest prevalences corresponded to penicillin (45%), aminoglycoside (40%) and non-extended spectrum cephalosporin (39%) classes. Multidrug resistance (MDR) was observed in 53.4% of the resistant isolates. No resistance to colistin was found. Production of ESBL/AmpC enzymes was detected in 5% of E. coli. Shiga toxin-producing E. coli were not observed, enteropathogenic E. coli were identified in only 12% of them, and ExPEC were found in 25%. Dog faeces can be a source of E. coli strains potentially presenting a threat to humans through their virulence factors or AMR. The non-hygienic keeping of animals may increase the risk of colonisation of such pathogens in humans.

Idioma: Inglés

DOI: 10.1556/004.2020.00013

Año: 2020

Publicado en: ACTA VETERINARIA HUNGARICA 68, 1 (2020), 1-7
ISSN: 0236-6290

Factor impacto JCR: 0.955 (2020)

Categ. JCR: VETERINARY SCIENCES rank: 101 / 146 = 0.692 (2020) - Q3 - T3

Factor impacto SCIMAGO: 0.395 - Veterinary (miscellaneous) (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/A05-17R
Financiación: info:eu-repo/grantAgreement/ES/DGA/A13-17R
Financiación: info:eu-repo/grantAgreement/ES/MICIU/FPU14-02035

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Genética (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)