Transcriptomic analysis of Escherichia coli MG1655 cells exposed to pulsed electric fields

Chueca, B. (Universidad de Zaragoza) ; Pagán, R. (Universidad de Zaragoza) ; García-Gonzalo, D. (Universidad de Zaragoza)

Resumen: DNA microarrays were used for the first time to study the mechanism of bacterial inactivation by pulsed electric fields (PEF). Escherichia coli MG1655 cells were PEF-treated for 50 pulses (0.08 Hz) at 20 kV/cm (2.10 kJ/kg per pulse) in buffer at pH 4.0 (100 μs PEF treatment time). After this PEF treatment, 47 genes demonstrated transcriptional differences. Among the 20 up-regulated genes, we found cytochrome bo oxidase genes (cyoB, cyoC, cyoD) and heme O synthase (cyoE), succinate dehydrogenase (sdhCDAB) operon, and transcriptional repressors of bet genes and chromosomal ars operon (betI and arsR, respectively). Gene class testing showed relevance of the tricarboxylic acid (TCA) cycle pathway, confirming cell requirement for energy and reducing power after PEF treatments. Transcriptomics after this PEF treatment were compared with cell transcriptome after a heat treatment using the same experimental setup, revealing a weaker cell response to PEF, which mainly involves components and functions directly associated with the cytoplasmic membrane. Resistance experiments confirmed results observed by transcriptomics, showing the lack of cross-resistance phenomena by PEF treatments.

Idioma: Inglés

DOI: 10.1016/j.ifset.2014.09.003

Año: 2015

Publicado en: INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES 29 (2015), 78-86
ISSN: 1466-8564

Factor impacto JCR: 2.997 (2015)

Categ. JCR: FOOD SCIENCE & TECHNOLOGY rank: 18 / 125 = 0.144 (2015) - Q1 - T1

Factor impacto SCIMAGO: 1.675 - Chemistry (miscellaneous) (Q1) - Industrial and Manufacturing Engineering (Q1) - Food Science (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2012-32165

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Tecnología de Alimentos (Dpto. Produc.Animal Cienc.Ali.)