miR482f and miR482c-5p from edible plant-derived foods inhibit the expression of pro-inflammatory genes in human THP-1 macrophages

Díez-Sainz, Ester ; Lorente-Cebrián, Silvia (Universidad de Zaragoza) ; Aranaz, Paula ; Amri, Ez-Zoubir ; Riezu-Boj, José I. ; Milagro, Fermín I.

Resumen: Edible plants can exert anti-inflammatory activities in humans, being potentially useful in the treatment of inflammatory diseases. Plant-derived microRNAs have emerged as cross-kingdom gene expression regulators and could act as bioactive molecules involved in the beneficial effects of some edible plants. We investigated the role of edible plant-derived microRNAs in the modulation of pro-inflammatory human genes.MethodsMicroRNAs from plant-derived foods were identified by next-generation sequencing. MicroRNAs with inflammatory putative targets were selected, after performing in silico analyses. The expression of candidate plant-derived miRNAs was analyzed by qPCR in edible plant-derived foods and their effects were evaluated in THP-1 monocytes differentiated to macrophages. The bioavailability of candidate plant miRNAs in humans was evaluated in feces and serum samples by qPCR.ResultsmiR482f and miR482c-5p are present in several edible plant-derived foods, such as fruits, vegetables, and cooked legumes and cereals, and fats and oils. Transfections with miR482f and miR482c-5p mimics decreased the gene expression of CLEC7A and NFAM1, and TRL6, respectively, in human THP-1 monocytes differentiated to macrophages, which had an impact on gene expression profile of inflammatory biomarkers. Both microRNAs (miR482f and miR482c-5p) resisted degradation during digestion and were detected in human feces, although not in serum.ConclusionOur findings suggest that miR482f and miR482c-5p can promote an anti-inflammatory gene expression profile in human macrophages in vitro and their bioavailability in humans can be achieved through diet, but eventually restricted at the gut level.

Idioma: Inglés

DOI: 10.3389/fnut.2023.1287312

Año: 2023

Publicado en: Frontiers in Nutrition 10 (2023), 1287312 [15 pp.]
ISSN: 2296-861X

Factor impacto JCR: 4.0 (2023)

Categ. JCR: NUTRITION & DIETETICS rank: 31 / 114 = 0.272 (2023) - Q2 - T1

Factor impacto CITESCORE: 5.2 - Endocrinology, Diabetes and Metabolism (Q2) - Food Science (Q2) - Nutrition and Dietetics (Q2)

Factor impacto SCIMAGO: 0.828 - Food Science (Q1) - Nutrition and Dietetics (Q2) - Endocrinology, Diabetes and Metabolism (Q2)

Financiación: info:eu-repo/grantAgreement/ES/ISCIII/CB12-03-30002
Financiación: info:eu-repo/grantAgreement/ES/MINECO-FEDER/BFU2015-65937-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/PID2022-141766OB-I00
Financiación: info:eu-repo/grantAgreement/ES/MINECO/RTI2018-102205-B-I00

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Fisiología (Dpto. Farmac.Fisiol.y Med.L.F.)